Composite

Part:BBa_K1647005:Design

Designed by: Yun Xue   Group: iGEM15_WHU-China   (2015-09-15)

J23100+Cph8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 427
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used a strong promoter and strong RBS to produce high levels of expression of the chimeric protein Cph8.

Source

Assembled from parts in the previous distribution kits.

References